P01_UoM_CAIX

Project

Project ID: P01_UoM_CAIX

User

Name: Sergi Celis
Institution: University of Manchester

Protein

ID (UniProt code): Q16790
Source: Human Carbonic Anhydrase IX (38-414), His Tag(CA9-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414
Supplier: Acro Biosystems
Stock (µg): 50.0
State: lyophilised
Protein Buffer PB: 20 mM MES, 100 mM NaCl, pH 6.5, trehalose as protectant
Reconstitution solvent: water
Reconstitution volume (µL): 125.0

Selection P01_UoM_CAIX_SELE_1_1

Protein

Input (µg): 4.8
Volume (µL): 12

Immobilisation

Beads Source: Invitrogen_Dynabeads™ His-Tag Isolation and Pulldown_Catalog number 10103D
Beads (µg): 160
Buffer IB: PB + 0.01% Tween-20
Incubation (rpm): 1100
Temperature (°C): 4
Time (minutes): 30

Blocking

Buffer BLB: IB + 0.2 mg/mL herringsperm DNA
Volume (µL): 100

DEL

ID(s): DEL1-115 spin-filter-purified
Input (fmol DNA): 250
Volume (µL): 1
Pre-clearing: yes
Incubation (rpm): 1100
Temperature (°C): 23
Time (minutes): 45

Washing

Buffer: IB
Volume (µL): 100
Frequency Washes: 10
Number of washes: 8

Elution

Buffer: water
Volume (µL): 20
Number of elutions: 2
Incubation (rpm): 1100
Temperature (°C): 80
Time (minutes): 5

PCR

Buffer: 5 mM Tris/HCl, pH 8.5
Volume (µL): 30
DNA amount (ng): 600
Gel image (purified amplicons): Selection_1_Gel_Image_Example

Results

FASTQ Files - ID: 40-1111632059_P01_UoM_CAIX_SELE_1_1
FASTQ Files - Reads: 40321
FASTQ Files - Raw_fastqs: SELE25-2_R2_001.fastq
FASTQ Files - Rawdata_qc: SELE26-2_R2_001.fastq
BB number of counts: Bck24_R1_001.fastq

Compound Result 1

Structure & Validation

Confirmed Structure: yes
On-DNA Validation: SELE24-1_R1_001.fastq
Off-DNA Validation: SELE25-2_R2_001.fastq

Smiles

O=S(C1=CC=C(B(O)O)C=C1)(N)=O

Compound Result 2

Structure & Validation

Confirmed Structure: no
On-DNA Validation: SELE25-2_R2_001.fastq
Off-DNA Validation: Bck24_R1_001.fastq

Smiles

OC[C@@H](O1)[C@@H](O)[C@H](O)[C@@H]2[C@@H]1c3c(O)c(OC)c(O)cc3C(=O)O2

Compound Result 3

Structure & Validation

Confirmed Structure: no
On-DNA Validation: DNA Encoded Library MTA 1.3
Off-DNA Validation: SELE26-2_R2_001.fastq

Smiles

CC1=NN(C(C)=C1C2=CC=C(S(N)(=O)=O)C=C2)CC(NCC3=CN=CC=C3)=O