The first step is to contact us via email at sam.butterworth@manchester.ac.uk and sergi.celis@manchester.ac.uk, briefly explaining your project and the protein/s you would like to screen. We aim to reply within two working days to confirm the receipt of your request and start conversations about the project.
To use this resource it is required that the user provides a few milligrams of the protein with a His-Tag or similar, so we can immobilise it on beads, and ideally with evidence that the expressed protein is active or correctly folded. In order to test several conditions, it would be advantageous to have the total amount of protein aliquoted in lyophilised form so it does not go through too many freeze-thaw cycles and we can test different buffers, if appropriate.
We will also require information about the protein such as compatible/incompatible buffers and additives beforehand to assess your request.
Please download the material transfer agreement from the link below and send it signed to us.
